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. Author manuscript; available in PMC: 2018 Aug 4.
Published in final edited form as: J Org Chem. 2017 Jul 14;82(15):8199–8205. doi: 10.1021/acs.joc.7b00845

Figure 3.

Figure 3

Fluorescent detection of K+ depletion in cells. (A) CHO cells transiently transfected with empty pCDNA3.1 plasmid (−) or Shaker-IR DNA labeled with 50 μM KNIR-1. Yellow dotted circles indicate the patch clamped cell in the field of view; fluorescent image scale bars represents 10 μm. Voltage-clamp fluorometry traces for (B) pCDNA3.1 (−) and (C) Shaker-IR: cells were held at −80 mV and currents (I) were elicited from 2-s command voltages from 0 to 100 mV in 20-mV increments. Total cellular fluorescence was simultaneously collected at 10 Hz by 10-ms excitation at 635 nm; F0 is the average fluorescence intensity of the first ten data points before the test depolarization. Voltage clamp fluorometry scale bars are for both (B) and (C); dotted line indicates zero current or change in fluorescence.