Fig. 4.

The −574 to −550 and −490 to −478 fragments in the −574 to −455 region are responsive to R. solani. a Schematic diagram of the −574 to −455 region (pCXGFP delA) and the five deleted derivatives (pCXGFP delB to pCXGFP delF) used to express GFP in tobacco leaves. b GFP fluorescence assay of young and expanded symmetrical NB leaves infiltrated with pCXGFP delA or its derivatives after R. solani strain YWK196 infection for 24 h. Bars = 5 mm. c Quantitative fluorometric assay of NB leaves. The fluorescence value was calculated relative to CK of pCXGFP delA. The 35S minimum promoter was used as the negative control. Asterisks indicate statistically significant differences, as determined by Student’s t-tests (*P < 0.05)