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. 2017 Dec 5;16:221. doi: 10.1186/s12934-017-0836-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Mining of propeptides from sequenced Lactococcus genome assemblies. a Alignment of native Lactococcus proteins with USP45-LEISSTCDA sequence. Alignment was created in ESPript 3.0 [31]. b Comparison of isoelectric points and net charges of the three propeptides (PP1–PP3) and positive control LEISSTCDA (PC) propeptide