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. 2017 Oct 22;174(23):4345–4361. doi: 10.1111/bph.14035

Figure 1.

Figure 1

Effects of p53 activation on bile acid disposition‐related gene expression in primary hepatocytes of Trp53‐WT mice. The cells were incubated with Trp53 siRNA for 60 h and treated with 0.5 μM doxorubicin (Dox) for the last 48 h. mRNA and protein were measured by qRT‐PCR and Western blot analysis respectively. (A) Western blots of p53 and GAPDH proteins and the corresponding quantification of p53 relative to GAPDH. (B–N) mRNA expression of Cdkn1a, Slco1b2, Slc10a1, Cyp7a1, Cyp3a11, Cyp2b10, Ugt1a1, Sult2a1, Abcb11, Slc51b, Abcc2, Abcc3 and Abcc4. Data are the mean ± SEM (n = 5). *P < 0.05.