Figure 5.
RhoA inhibition reverses carbachol‐induced bronchoconstriction in a dose‐dependent manner in hPCLS. Measurement of bronchodilation concentration–response to Rhosin in hPCLS. Airways were preconstricted with carbachol (10−8–10−4 M) before dilation with Rhosin or formoterol (10−10–10−4 M). Data were normalized to forskolin stimulation (10 μM) that was given after the final dose of formoterol or Rhosin. Each data point is expressed as mean ± SEM. Each group contains two airways from each of the three donors (total of six airways).