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. 2017 Dec 4;13:377. doi: 10.1186/s12917-017-1305-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Dispersion plot of ELISA reactivity versus BVDV-1 recombinant E2 (Y axis) and BVDV-2 recombinant E2 (X axis). Diagonal and envelope lines represent equal reactivity and cut-off discrimination, respectively. Panel a: Reactivity of pooled milk samples. Strain specific control sera are shown as white triangles. White dots denote pool milk samples reactive against BVDV-1 antigen. Grey dots denote indeterminate samples. Black dots denote pools from a single dairy farm reactive against BVDV-2 antigen. Panel b: Spot test on young animals. Grey dots denote negative or indeterminate samples. Black dots denote individual serum samples reactive against BVDV-2 antigen. Black squares denote individual serum samples from a BVDV-1 positive farm