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. Author manuscript; available in PMC: 2017 Dec 5.
Published in final edited form as: Exp Cell Res. 2015 Sep 25;344(2):167–175. doi: 10.1016/j.yexcr.2015.09.014

Figure 3.

Figure 3

Double immunofluorescence staining analyses of Aqp3 and AE1 showing less PCs and more α-ICs in Dot1lAC than in control mice. (A) Representative IF images showing staining of Aqp3 (red) and AE1 (green) as PC and α-IC markers, respectively, in the outer medulla of adult Dot1lf/f and Dot1lAC mice. The insert shows the enlarged boxed areas. Scale bar: 100 μM and 25 μM for insert. (B–D). Bar graphs showing the relative percentages of the 4 groups based on the staining of the two markers in the CNT/CD cells in the cortex (B), outer medulla (C), and inner medulla (D) of Dot1lf/f and Dot1lAC mice. In all cases, *P < 0.05 vs. Dot1lf/f.