Study design of cell-free patient-specific nanofiber tissue engineered vascular
graft. A and B, The dimension and shape of the thoracic inferior vena cava
(IVC) was measured from angiography before surgery in the
sheep model. C, An electrospinning mandrel was modeled by computer-aided design
and subsequently 3D-printed. D, The nanofiber scaffold was electrospun onto the
3D-printed mandrel. E and F, A patient-specific cell-free nanofiber
tissue-engineered vascular graft (TEVG) was implanted as an IVC interposition
conduit in the sheep model. G and H, Scanning electron microscope images of the
scaffold (G, 500×; H, 4000×). I, Intraoperative picture of the
implanted TEVG. 3D, 3-dimensional.