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. Author manuscript; available in PMC: 2017 Dec 5.
Published in final edited form as: J Mol Cell Cardiol. 2017 Mar 3;105:59–69. doi: 10.1016/j.yjmcc.2017.03.001

Fig. 6.

Fig. 6

Neuregulin-1β treatment induces phosphorylation of ErbB2 receptor in human cardiac fibroblasts. A, Western blot analysis of protein lysates of NHCV fibroblasts treated for 30 min with 30 ng/mL of NRG-1β. Bands shown are 185 kDa and were detected using an anti-rabbit antibodies that recognize phosphorylated ErbB2 (pErbB2) at tyrosine position 1289 (Tyr1248) and pan ErbB2. A representative blot is shown. B, Western blot analysis of protein lysates of NHCV fibroblasts treated for 30 min with 30 ng/mL of NRG-1β. Bands shown are 185 kDa and were detected using anti-rabbit antibodies that recognize phosphorylated ErbB2 (pErbB2) at tyrosine positions 1221 and 1222 (Tyr1221/1222), and pan ErbB2. C, Graphical representation showing densitometry analysis result for blots shown in A and B. Asterisks represent significance (n= 3, *p < 0.05 for NRG-1β-treated cells compared to controls).