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. 2017 Nov 13;114(48):E10349–E10358. doi: 10.1073/pnas.1710298114

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Copyright © 2017 the Author(s). Published by PNAS.

This is an open access article distributed under the PNAS license.

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Fig. 4. — Stem mismatch breaks the correlation between dicing and silencing. (A) Schematic of the RNAi pathway. Small RNAs with varying numbers of stem mismatches are applied to dicing and silencing measurement. (B) Dicing result of U27 with no mismatch and U27_4M with four mismatches in the stem. (C) Quantification of dicing kinetics. (D) mRNA histogram of silencing induced by different stem-mismatched RNA substrates. (E) Analysis of the histogram in D for the four stem-mismatch variants. (F) Silencing efficiency plotted against dicing rate for stem-mismatch variants.