Fig. 1.

IFNγ levels in the PBMCs of patients with colon cancer of different stages. (A) PBMCs were stimulated, and IFNγ secretion was detected using a human IFNγ ELISA kit. (B) RSK2⁻ lymphoblasts showed lower IFNγ mRNA levels compared with RSK2⁺. GM09621 (RSK2⁺) and GM03317 (RSK2⁻) were treated with PMA and ionomycin or untreated. Gene expression of Ifnγ was analyzed by PCR. Gapdh served as an internal control. (C) Analysis of IFNγ populations in the spleens and lymph nodes of WT and RSK2 KO mice. The average fluorescent intensity of IFNγ expression was analyzed by flow cytometry.