Fig. 2.

The rate of colon cancer liver metastasis is significantly increased in RSK2 KO mice. (A) Representative photographs of spleen and liver metastases (Mets) of WT and RSK2 KO mice. (B) Immunohistochemical analysis of tumor tissues. Tumor tissues were stained with H&E or a PCNA antibody. The number of PCNA⁺-stained cells was significantly higher in the RSK2 KO group compared with the WT group. *P < 0.05. (C) Average liver metastasis weight of RSK2 KO mice was significantly higher than that of WT at 11 d after tumor implantation (Left; *P < 0.05, t test). Average spleen weight of RSK2 KO mice was significantly higher than that of WT spleen at 11 d after tumor implantation (Center; *P < 0.05, t test). The average body weight of WT and RSK2 KO mice was not different (Right).