Fig. 5.

Distribution of cargo proteins and Sec16 in cells lacking Tango1. White boxes in A–D are representative fields magnified in A′–A‴, B′–B″, C′–C‴, and D′–D‴, respectively. (A and B) Confocal projections of terminal cells expressing mCD8mCherry under SRF-gal4, endogenously tagged Dpy-YFP (A′ and B′) and stained for Sec16 (A′′ and B′′). While in control cells Dpy-YFP is seen at the luminal membrane (A–A′′′), in cells lacking tango1 Dpy-YFP shows a cytoplasmic distribution that partially overlaps with Sec16 (B–B′′′). (C and D) Confocal projections of terminal cells expressing GFP under SRF-gal4 and stained for Sec16 and Crb. While in control cells Crb localizes to the luminal membrane (C–C′′′), in cells lacking tango1 Crb shows a cytoplasmic distribution that partially overlaps with Sec16 (D–D′′′). (Scale bars: A–D, 10 μm; A‴–D‴, 2 μm.)