Fig. 6.

Effect of loss of Tango1 and Dpy on Sec16 distribution. (A–D) Tango1 (A′–D′) and Sec16 (A″–D″) in terminal cells expressing GFP under SRF-gal4. In control (A–A‴) and dpy-IR cells (C–C‴), Sec16 particles are homogeneous in size and fluorescence intensity; in tango1-IR cells, Sec16 particle size and fluorescence intensity is variable (B–B‴), and this variability is not altered by simultaneously removing Dpy (D–D‴). Insets in (A″–D″) are magnifications of representative regions, indicated by the white squares. (E and F) Variance of Sec16 particle size (E) and Sec16 fluorescence intensities (F). Control, n = 5; tango1-IR, n = 4; dpy-IR, n = 4; tango1-IR+dpy-IR, n = 5. Significance was determined using one-way ANOVA and Sidak’s multiple comparisons test. (Scale bars: 10 μm; Insets, 2 μm.)