Table 1.

Binding affinity of B. subtilis Fur to its target promoters

Order of induction	Promoter	Kd*(nM)
Early	fpbN	5.6 ± 0.5
	efeU	1.5 ± 0.3
	fecC	3 ± 0.5
Middle	ykuN	2.1 ± 0.4
	dhbA	2.1 ± 0.5
	fhuB	0.9 ± 0.2
	feuA	0.8 ± 0.2
Late	fsrA	0.6 ± 0.2
	fbpA	0.6 ± 0.1
	fbpC	0.5 ± 0.1

^*K_d values were calculated using GraphPad Prism 5 (mean ± SD, n = 3) (SI Appendix, Figs. S7 and S8).