Fig. 5.

Controlling chemotherapeutic drug delivery in F98 rat glioma model. (A) Schematic illustration of the experimental protocol. (B) For treatment planning, an axial representative T2-weighted MR image was acquired on the sonication day for visualizing the anatomical structures and locations of the bilateral tumors. Plus refers to the targeted location. (C) Representative fluorescent imaging for TB (Left) and DOX (Right). (D) Contrast-enhanced T1-weighted MR images acquired posttreatment for assessing BTBD/BBBD on the tumor (Left) and hippocampus (Right) targets. The axial images were reformatted to show sagittal (Right) and coronal (Bottom) planes. The arrows indicate the direction toward the ventral surface. All MRI and fluorescent images shown in Fig. 5 were obtained from the same animal. (E) MRI contrast enhancement (in percent, compared with the baseline images acquired before contrast agent administration) evaluations at three time points post–contrast agent injection. Fluorescent intensities of TB (F) and DOX (I) in control and sonicated locations were compared. Error bars represent SDs. The relationships between the delivered TB concentration (right axis)/fluorescent enhancement (left axis) and total HE for (G) tumor and (H) hippocampus targets were assessed (solid lines, piecewise linear regression; dotted lines, 95% confidence intervals). The relationships between delivered DOX concentrations (right axes)/fluorescent enhancement (left axes) and total HE for (J) tumor and (K) hippocampus targets were also evaluated.