Table 1.

Dissociation constants for NANP/NVDP repeat-containing peptides obtained from ITC affinity measurements

Binding parameter	(NPNA)3	(NPNA)2	NPNVDPNANPNV	DPNANPNVDPNA
Fab311
No. of sites	0.74 ± 0.02	1.50 ± 0.08	1.24 ± 0.02	1.20 ± 0.03
Kd, μM	0.305 ± 0.060	0.296 ± 0.019	1.787 ± 0.189	1.368 ± 0.390
∆H, cal/mol	−29,823 ± 261	−16,363 ± 261	−18,657 ± 378	−20,200 ± 1,078
∆S, cal−1⋅mol−1⋅degree−1	−70 ± 1	−25 ± 1	−36 ± 2	−41 ± 4
Fab317
No. of sites	1.27 ± 0.02	1.41 ± 0.05	1.72 ± 0.13*	1.64 ± 0.05
Kd, μM	0.078 ± 0.016	0.173 ± 0.008	12.091 ± 2.488*	0.448 ± 0.068
∆H, cal/mol	−15,700 ± 225	−12,090 ± 221	−9,657 ± 426*	−9,387 ± 152
∆S, cal−1⋅mol−1⋅degree−1	−20 ± 1	−10 ± 1	−10 ± 2*	−2 ± 1

All values are the average and SD of triplicate experiments except for those with an asterisk (*), which are from duplicate experiments.