Figure 3.

Variation in green fluorescent protein (GFP) expression and alkaline phosphatase (AP) activity in the colonies in the culture media with different pH conditions. A, Phase‐contrast (phase), expression of GFP, and AP activity in each culture dish were examined in the colonies that were cultured under different pH conditions for 17 days for the induction of somatic cell reprogramming in mice. B, The morphology of the colony formation of mouse embryonic stem cells under different pH conditions in the presence of leukemia inhibitory factor (in particular, the GFP signals of the Oct4 reporter and phase‐contrast images) were examined. ESC, embryonic stem cells; LIF, leukemia inhibitory factor