Table 1.

Sequences of the primers for the reverse transcription–polymerase chain reaction

Gene	Primer sequence	Anealing temperature (°C)	Product size (bp)	Source
Mixl1	5′‐GCACGTCGTTCAGCTCGGAGC‐3′5′‐AGTCATGCTGGGATCCGGAACGTGG‐3′	55	305	Jackson et al. (2010)27
Nestin	5′‐ GGAGAGTCGCTTAGAGGTGC‐3′5′‐AGGTGCTGGTCCTCTGGTAT‐3′	55	375	NM_016701.3
T	5′‐TGCTGCCTGTGAGTCATAAC‐3′5′ TCCAGGTGCTATATATTGCC‐3′	55	948	Jackson et al. (2010)27
Sox2	5′‐TAGACTGCACATGGCCCAGCACT‐3′5′‐TTGCCTTAAACAAGACCACGAAA‐3′	55	778	NM_011443.3
Oct3/4	5′‐AAGTGCCCGAAGCCCTCCCTACAG‐3′5′‐CAGAGGGAAAGGCCTCGCCCTCAG‐3′	55	289	NM_001252452.1
Gapdh	5′‐ACGGCACAGTCAAGGCAGAG‐3′5′‐GTGATGGCGTGGACAGTGGT‐3′	55	376	NM_001289726
For real time
Mixl1	5′‐CAGTTGCTGGAGCTCGTCTT‐3′5′‐TCCGGAACGTGGTTCACATC‐3′	60	266	NM_013729.3
Nestin	5′‐GGGGCTACAGGAGTGGAAAC‐3′5′‐GACCTCTAGGGTTCCCGTCT‐3′	60	213	NM_016701.3
T	5′‐GGCTGGGAGCTCAGTTCTTT‐3′5′‐TGTCCACGAGGCTATGAGGA‐3′	60	179	NM_009309.2
Sox2	5′‐GATCAGCATGTACCTCCCCG‐3′5′‐TCCTCTTTTTGCACCCCTCC‐3′	60	212	NM_011443.3
Oct3/4	5′‐CCAATCAGCTTGGGCTAGAG‐3′5′‐CCTGGGAAAGGTGTCCCTGT‐3′	60	130	NM_001252452.1
Gapdh	5′‐CGTGTTCCTACCCCCAATGTG‐3′5′‐TGTCATCATACTTGGCAGGTTTC‐3′	60	73	NM_001289726