Figure 4.

Biofilm Formation of the Hyperfilamentous mutant Δhog1 under flow: (A) Representative darkfield images of biofilm formation under flow are shown for wild-type (top) and Δhog1 (bottom) cells grown at room temperature at 2, 8 and 18 h of growth. Scale bars indicate 50 µm. Arrow indicates direction of media flow for every image; (B) The total biomass within the imaging region (determined by densitometry analysis), the rate of cell attachment, and the detachment rate normalized to the biomass over time are shown for Δhog1 biofilms (red) and wild-type at room temperature biofilms (black). Data are means of n ≥3 experiments.