Table 1.

Quantification of real-time biofilm formation of Candida albicans under dynamic flow.

Strain (Temperature)	Attachment Rate 1	Cell–Cell Adhesion 2	Biomass 3 (×106)	Biofilm Growth Rate 4 (×106)	Normalized Detachment Rate 5 (×10−8)
CAI4 (RT)	2.43	0.46	190.5	11.43	4.29
CAI4 (37 °C)	9.27	0.62NS	ND	ND	ND
Δhog1 (RT)	1.71	0.41NS	969.4	73.75	2.67

Each value represents a mean or best-fit slope of n ≥3 experiments. NS indicates no significant difference between each strain compared to CAI4 at room temperature (RT). All other values were significant (p < 0.05). ND indicates that the value was not determined. ¹ Attachment Rate represents the average coverage area of newly attached cells/imaging area/h. Values represent a change in coverage area of attached cells as a percentage of total area over time. ² Cell–Cell Adhesion was calculated as the average events/total substrate adhesion events between 1 and 2 h during the attachment phase. ³ Biomass was calculated as the cumulative light intensity of the imaging area (in gray values), measured at 18 h. ⁴ Biofilm Growth Rate was calculated as the best fit slope of the biomass over time in gray values/h obtained by linear regression. ⁵ Normalized Detachment Rate was calculated as the average total detachment rate (average area of newly detached cells/total imaging area/h)/biomass. Values indicate the probability for any individual cell to detach from the surface or biofilm.