Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 4;214(12):3775–3790. doi: 10.1084/jem.20161868

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Veselits et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Selective defect in late B cell development in Igβ^KΔR mice. (A–C) BM cells from WT and Igβ^KΔR mice were isolated, stained with antibodies specific for B220, CD43, and IgM, and analyzed by flow cytometry (A and B). Total numbers of each population (C) are provided for WT (red) and Igβ^KΔR (blue) mice; error bars indicate mean ± SD. *, P = 0.0167; **, P = 0.0022 (n = 3). (D) Thymic CD3⁺ lymphocytes were analyzed by flow cytometry for CD4 and CD8 expression (n = 3 mice). (E and F) Splenic B cells from the indicated mice were stained with antibodies specific for B220, IgM, IgD, CD21, CD23, and CD93 and then analyzed by flow cytometry. Representative flow cytometric plots are shown in E, and total numbers of each population from WT (red) and Igβ^KΔR (blue) are shown in F. *, P = 0.0046; **, P = 0.0021; ***, P = 0.0044; †, P = 0.0009; ‡, P = 0.0012; §, P = 0.0033 (n = 4 mice per condition). (G) Flow cytometry of WT (red) and Igβ^KΔR (blue) BM immature and mature B cells stained for Igκ and Igλ (n = 3). (H and I) BM from WT CD45.1 mice was mixed 50:50 with WT CD45.2 (red) or Igβ^KΔR CD45.2 (blue) BM and then transferred into irradiated WT CD45.1 mice. After 8–9 wk, BM (*, P = 0.006; **, P = 0.009; H) and spleen (*, P = 0.003; **, P = 0.002; I) were analyzed by flow cytometry. Each point represents one mouse. Horizontal lines represent means. MZ, marginal zone. Fol, follicular B cells.