Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 4;214(12):3553–3563. doi: 10.1084/jem.20170180

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Yu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — USP1–UAF1 complex enhances IRF3 activation. (A and B) HEK293T cells were transfected with the indicated adaptors along with IRF3 or NF-κB reporter plasmid and UAF1 or USP1 plasmid, and luciferase activity was analyzed. (C and D) Western blot analysis of the indicated phosphorylated signaling proteins in peritoneal macrophages incubated with DMSO or 30 µM ML323 and then stimulated with SeV (C) or ISD (D). (E–H) Western blot analysis of the indicated phosphorylated signaling proteins in peritoneal macrophages transfected with control siRNA, UAF1 siRNA, or USP1 siRNA and then stimulated with LPS (E), poly(I:C) (F), SeV (G), or ISD (H). Data are representative of three experiments (mean ± SD of six samples in A and B; **, P < 0.01; by Student’s t test.). Similar results were obtained in three independent experiments in C–H. Ctrl, control.