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. 2017 Dec 4;214(12):3543–3552. doi: 10.1084/jem.20170468

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© 2017 Mu et al.

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Figure 3. — Enhanced AID Serine 38 phosphorylation does not increase CSR. (A) Anti-pS38 and anti-AID immunoblot of AID purified with anti-AID antibody from lysates of B cells from WT, AID^S38A, or AID^−/− mice. Cells were cultured with IL-4 and LPS for 72 h and treated with 10 mM LiCl (Li⁺) or NaCl (Na⁺) for 12 h before analysis. (B) Representative triplicate FACS plots of CSR to IgG1 in WT and AID^S38A B cells from A. Relative percentage of cells expressing IgG1 is in each plot. (C) Summary of CSR to IgG1 in activated B cells from n = 6 individual experiments each in triplicate. Error bars are SE. P-value (*, P < 0.001) was determined by a two-tailed t test assuming unequal variance.