Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 4;214(12):3543–3552. doi: 10.1084/jem.20170468

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Mu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — AID Serine 38 phosphorylation promotes Myc/Igh translocations. (A) Schematic for the Myc/Igh translocation assay. PCR amplification primers are represented by black arrows and Southern probes by gray bars. Closed circles denote centromeric locations on the chromosomes. (B) Representative translocation assay Southern blots with Myc and Igh probes are displayed; each lane contains the DNA content of 1×10⁵ genomes. B cells from WT or AID^S38A mice cultured with IL-4 and LPS were treated with 10 mM NaCl or LiCl for 12 h before analysis. (C) Total translocation frequency summary from n = 4 independent experiments. P-values (*, P < 0.01) determined using two-tailed Fisher’s exact test (WT Na⁺ vs. AID^S38A Na⁺ or Li⁺, both P > 0.5).