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. 2017 Dec 4;214(12):3627–3643. doi: 10.1084/jem.20170545

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© 2017 Singh et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 1. — Small RNA sequencing expression analysis of miRNAs in ILC2s and T cells. (A) Pearson correlation coefficients for global miRNA expression profiles among all samples. Sample groups include ILC2s isolated from naive mouse lung (ILC2), ILC2s isolated from mouse lung after IL-33 treatment (ILC2 IL33), ILC2 cultured in vitro for 10 d (ILC2 in vitro), naive CD4 T cells (CD4), T_H2 cells isolated from BAL of mice with AAs (T_H2 AA), and T_H2 cells polarized in vitro (T_H2 in vitro). (B) Unsupervised hierarchical clustering of all DESeq2 differentially expressed miRNAs. A list of individual miRNAs in each subgroup is included in Table S2. (C) Heat map of log₂ normalized read counts and fold changes of miRNAs significantly (adjusted P < 0.01) up- or down-regulated in ILC2s after culture in vitro with IL-33, TSLP, and IL-7. Values for naive CD4 T cells and T cells after 5 d of T_H2 polarizing culture are also provided for comparison. (D) Quantitative PCR confirmation of RNA sequencing data for the indicated miRNAs. Bars represent mean ± SEM. P-values were calculated with Student’s t test.