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. 2017 Dec 4;214(12):3627–3643. doi: 10.1084/jem.20170545

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© 2017 Singh et al.

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Figure 2. — Severe reduction of lung ILC2s in Red5 Dgcr8^Δ/Δ mice. (A) Contour plots depict the gating strategy for lung ILC2s in Dgcr8^Δ/+ and Dgcr8^Δ/Δ mice. Total ILC2s are identified as live CD45⁺CD90⁺lin^–CD4^–CD25⁺ cells. Among total ILC2s, tdTomato (Red5) fluorescence indicates Cre expression, and YFP expressed from the R26-YFP reporter indicates Cre activity. (B) Flow cytometric analysis of ST2 median fluorescence intensity (MFI) in the total ILC2s of Dgcr8^Δ/+ and Dgcr8^Δ/Δ lung. (C–F) Flow cytometric quantification of total ILC2s (C), YFP⁺ ILC2s (D), Red5⁺ YFP^–ILC2s (E), and Red5^– YFP^–ILC2s (F) in the lung of Dgcr8^Δ/+ and Dgcr8^Δ/Δ mice. Bar graphs show ILC2 frequency as percentage of live CD45⁺ cells (mean ± SEM). Each data point represents an individual mouse. Data were pooled from two independent experiments each with five to seven mice per group. P-values were calculated with Student’s t test.