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. 2017 Dec 4;214(12):3627–3643. doi: 10.1084/jem.20170545

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© 2017 Singh et al.

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Figure 5. — The miR-17∼92 cluster promotes ILC2s proliferation. (A) Microscopic view of tdTomato fluorescence in Red5⁺ ILC2s. Bars, 200 µm. (B) CyQUANT NF cell proliferation assay fluorescent quantification of 17∼92^Δ/Δ and 17∼92^+/+ ILC2s after culture for 4 d. (C) Quantification of Ki67⁺ 17∼92^Δ/Δ and 17∼92^+/+ ILC2s frequencies after culture for 3 d. (D and E) Apoptosis in 17∼92^Δ/Δ and 17∼92^+/+ ILC2s was assessed using Annexin V and DAPI staining on day 4 of culture. Bar graphs show the percentage of dead apoptotic ILC2s (DAPI⁺ Annexin⁺; D) and live apoptotic ILC2s (DAPI⁻ Annexin⁻; E). NS, not significant. Data in A, B, D, and E are representative of at least 20, 4, and 2 independent experiments, respectively. Bars represent mean ± SEM. P-values were calculated with Student’s t test (B–E).