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. 2017 Dec 4;214(12):3627–3643. doi: 10.1084/jem.20170545

Figure 7.

Figure 7.

Cell-intrinsic defect in IL-13 and IL-5 production in 17∼92Δ/Δ ILC2s rescued by miR-19a. (A) 10-d experimental model of next-generation transfection of ILC2s. Each culture was started with 10,000–12,000 17∼92Δ/Δ ILC2s sorted from the pool of three to six mice and cultured with IL-33, TSLP, and IL-7. Cells were transfected on day 8 with control mimic (CM) or mimics of miR-19a, miR-18a, miR-17, and miR-92a and harvested on day 10. (B–D) Quantitative PCR (qPCR) quantification of Gapdh-normalized Il13, Il5, and Il6 mRNA from 17∼92Δ/Δ ILC2s transfected with CM or miR-19a, miR-18a, miR-17, and miR-92a mimic. (E–G) IL-13, IL-5, and IL-6 measured by cytometric bead array (CBA) in supernatants from 17∼92Δ/Δ ILC2s transfected with miR-19a or control miRNA mimic. Data are pooled from five independent experiments and are normalized to matched control mimic transfection in each biological replicate. Bars represent mean ± SEM. (H) qPCR quantification of Gapdh-normalized Ki-67 mRNA expression from 17∼92Δ/Δ ILC2s transfected with miR-19a or control miRNA mimic. Bars represent mean ± SEM. Data in B–D and H are pooled from two independent experiments. P-values were calculated with one-way ANOVA with Dunnett’s multiple comparison post hoc test (comparing each column to CM; B–D), paired Student’s t test on unnormalized data (E–G), or Student’s t test (H).