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. 2017 Dec 4;214(12):3627–3643. doi: 10.1084/jem.20170545

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© 2017 Singh et al.

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Figure 9. — miR-19 targets Tnfaip3 and Socs1 negatively regulate IL-13 and IL-5 expression in ILC2s. (A) IL-13 and IL-5 measured by cytometric bead array (CBA) in supernatants from 17∼92^Δ/Δ ILC2s transfected with siRNAs targeting Tnfaip3 or Socs1 or negative control siRNA (siNegCtl). (B) Quantitative PCR quantification of Il13 and Il5 mRNA, normalized to Gapdh mRNA. Each culture was started with 10,000 ILC2s sorted from pools of five to seven mice. Data are pooled from three independent experiments. P-values were calculated with paired Student’s t test.