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. 2017 Sep 19;45(21):e176. doi: 10.1093/nar/gkx834

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — A schematic graphic output from miRvial. miRvial provides graphic output to assist visual inspection and selection of genuine miRNAs. Shown is an example of a miRNA detected by miRvial. (A) The upper panel gives the number of candidate miRNAs miRvial reported; one in this case shown. The panel shows the unique genomic locus of the miRNA, along with the predicted RNA secondary structure represented in parentheses, and the folding energy in a negative value. The lines below show the aligned sequencing reads, followed by the number of reads (# reads), the length of the unique read (length), and the number of mappable loci of the unique read (#. loci). (B) The lower-left plot shows the hairpin structure, where putative mature miRNAs are highlighted in color (blue from 5p-arm and red from 3p-arm). (C) The lower-right plot shows the distribution of reads in the predicted precursor sequence.