Table 1.

Sequence Similarity Within the Gclust Clusters and the Alignments for Trees

Tree Name	Figure #	Gclust2012				Gclust2017R6				Merge
		Cluster #	# of seq	E-value	Identity	Cluster #	# of seq	E-value	Identity	Identity
ATS1	3B	7661	26	1.00E-80	0.452 ± 0.131	2193	17	1.00E-45	0.360 ± 0.103	0.405 ± 0.117
PlsX	3A	1241	117	1.00E-31	0.405 ± 0.131	not used				n/a
PlsY	3C	937	141	1.00E-22	0.420 ± 0.112	not used				n/a
ATS2 & PlsC	4	488	190	1.00E-10	0.250 ± 0.100	1090	26	1.00E-50	0.319 ± 0.098	0.270 ± 0.098
						5855	7	1.00E-50	0.487 ± 0.084
		4242	42	1.00E-28	0.420 ± 0.113	7159	6	1.00E-45	0.427 ± 0.082
LPP	6	1284	113	1.00E-60	0.453 ± 0.175	1174	25	1.00E-12	0.252 ± 0.070	0.231 ± 0.115
		2041	76	1.00E-16	0.260 ± 0.104
		7867	25	1.00E-35	0.433 ± 0.177
		7879	25	1.00E-80	0.545 ± 0.190
		10402	19	1.00E-50	0.348 ± 0.143
		10903	18	1.00E-50	0.519 ± 0.186
		32991	5	1.00E-45	0.339 ± 0.137
		49241	3	1.00E-60	0.545 ± 0.046
GPT4	S8	5749	33	1.00E-60	0.388 ± 0.184	838	30	1.00E-50	0.246 ± 0.091	0.260 ± 0.113
		6695	29	1.00E-45	0.247 ± 0.129
		16298	12	1.00E-25	0.639 ± 0.314
LPAAT7	5	4244	42	1.00E-99	0.405 ± 0.207	1066	26	1.00E-70	0.243 ± 0.063	0.302 ± 0.132
		6202	31	1.00E-50	0.392 ± 0.181
LPAAT9	S7	5766	33	1.00E-70	0.400 ± 0.195	1492	21	1.00E-70	0.355 ± 0.106	0.333 ± 0.162
		7815	25	1.00E-45	0.334 ± 0.171
Reference clusters for comparison (acyltransferases)
NCBI CPDF		cd07984	100	n/a	0.176 ± 0.043
		cd07989	100	n/a	0.173 ± 0.050
InterPro		IPR004552	100	n/a	0.371 ± 0.255
			100	n/a	0.264 ± 0.122
			1000	n/a	0.278 ± 0.108
Pfam		PF01553	100	n/a	0.156 ± 0.056
			100	n/a	0.150 ± 0.050

Note.—The original clusters from Gclust2012 and Gclust2017R6 were individually aligned. The average identity ± standard deviation was calculated over all sequence pairs within each cluster. “Merge” indicates the data for the actual sequence alignments used for the phylogenetic tree construction. Comparable data of homologous sequence family were obtained from NCBI Cluster of Protein Domain, InterPro, and Pfam, and the average identity score was calculated in a similar way. “E-value” is the threshold of E-value. “n/a” indicates not applicable. For Reference clusters, 100 or 1000 sequences were arbitrarily taken from the original data and analyzed.