Figure 5.

Blockage of icosameric αHL pore. (A) λ-DNA molecules were added to the trans-side after a purified DNA/αHL hybrid structure was incorporated into a DPhPC bilayer. (B) Electrical recording of a [21⁺]₂₀-icosameric hybrid pore that is stochastically blocked by a λ-DNA molecule at applied negative potential (–50 mV; shaded in red) and opened at applied positive potential (50 mV; shaded in blue). (C) Current-voltage dependence of an unblocked pore (blue circles) versus a blocked pore (orange triangles).