Figure 3.

Binding of aminoglycosides, G418 (A) and paromomycin (B), to the ‘on’ states of the m¹A1408-modified A site. Overviews (top), pseudo pairs between m¹A1408 and ring I (middle) and detailed interactions of aminoglycosides to the mutant A site (bottom) are shown. The m¹A1408, A1492 and A1493 residues are colored in orange, blue and red, respectively. In the top figures, local F_o-F_c omit maps calculated after removing aminoglycosides are drawn in dark blue at 3 σ contour level. In the middle figures, hydrogen atoms colored in white are added for a better understanding. Hydrogen bonds and C-H…O interactions are shown as bold and thin dashed lines with distances in Å, respectively.