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. 2017 Oct 10;45(21):12425–12440. doi: 10.1093/nar/gkx927

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Modulation of DNA polymerization PabPolB with Mg²⁺ or Ca²⁺ on primed-M13mp18 DNA template. (A) Structure of the primer-template mimic, consisting of a Cy5-labeled 32-mer primer annealed to the circular M13mp18 DNA template of 7249-nt in length. (B) Extension of the primed-M13mp18 DNA template at the indicated Mg²⁺ or Ca²⁺ concentrations. The starting primer (32-nt) and full-length product (7249-nt) are shown arrowed on the left. Product length is indicated under the gel lanes. (C) Extension of the primed-M13mp18 DNA template for the times shown above the gels (min) at fixed Mg²⁺ or Ca²⁺ concentrations (5 mM). The starting primer (32-nt) and full-length product (7249-nt) are shown arrowed on the left. Product length is indicated under the gel lanes.