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. 2017 Oct 10;45(21):12425–12440. doi: 10.1093/nar/gkx927

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Single nucleotide incorporation by PabPolB in the presence of Mg²⁺ or Ca²⁺. (A) Primer-template used for these experiments consists of a Cy5-labeled 26-mer primer annealed to a DNA template of 34-nt in length. Incorporation using PabPolB exo+ (B) or PabPolB exo− (C) at 5 mM Mg²⁺ or Ca²⁺ concentrations. 0 = no dNTPs added; + = all four dNTPs added; A, T, C and G = only dATP or dTTP or dCTP or dGTP added, respectively. The extension (%) for selected lanes is shown under the gels.