. 2017 Sep 13;45(21):e174. doi: 10.1093/nar/gkx811

Table 1. Targeting frequencies following electroporation of insertion vectors pI_hygGFP into WT JM8 (parental or subcloned N4 or F6 lines as indicated) and heterozygous mutant tm1a/⁺ (knockout first) ES cells, selection in hygromycin and screening/genotyping by long range-PCR on genomic DNA.

Targeted gene (MGI symbol)	ES cell line/allele used for targeting	ES cell clone ID	Number of colonies screened	% clones targeted	MGI name for pI_hygGFP targeted allele (tm2)
*Brd8*	JM8.N4		24	96	Brd8^tm1Wcs
	JM8.N4/Brd8 ^{tm1a(EUCOMM)Wtsi/+}	E10	22	100
*Cbx1*	JM8.N4/Cbx1^{tm1a(EUCOMM)Wtsi/+}	B01	24	45.8	Cbx1^tm1Wcs
*Ddx27*	JM8.N4		24	100	Ddx27^tm1Wcs
	JM8.F6/Ddx27 ^{tm1a(KOMP)Wtsi/+}	E01	6*	0
*Dot1l*	JM8 parental/Dot1l ^{tm1a(KOMP)Wtsi/+}	D02	24	54.2	Dot1l^tm1Wcs
*Epc2*	JM8 parental/Epc2 ^{tm1a(EUCOMM)Wtsi/+}	B12	24	45.8	Epc2^tm1Wcs
*Ing3*	JM8.N4		24	79	Ing3^tm1Wcs
	JM8.N4/Ing3 ^{tm1a(EUCOMM)Wtsi/+}	E07	16	81
*Jarid2*	JM8 parental/Jarid2 ^{tm1a(KOMP)Wtsi/+}	A08	24	62.5	Jarid2^tm1Wcs
	JM8 parental/Jarid2 ^{tm1a(KOMP)Wtsi/+}	E08	24	62.5
*Kdm4c*	JM8.N4		12	100	Kdm4c^tm1Wcs
	JM8.F6/Kdm4c ^{tm1a(KOMP)Wtsi/+}	D03	24	75
*Mier1*	JM8.N4		24	66.7	Mier1^tm1Wcs
	JM8.N4/Mier1 ^{tm1a(EUCOMM)Wtsi/+}	A04	24	66.7
*Phf20*	JM8.N4		24	62.5	Phf20^tm1Wcs
	JM8.N4/Phf20 ^{tm1a(EUCOMM)Wtsi/+}	F07	0	0
*Setdb1*	JM8.N4		16	100	Setdb1^tm1Wcs
	JM8.N4/Setdb1 ^{tm1a(EUCOMM)Wtsi/+}	D08	40	0
*Smyd5*	JM8.N4		16	62.5	Smyd5^tm1Wcs
	JM8.N4/Smyd5 ^{tm1a(EUCOMM)Wtsi/+}	G01	24	25
*Supv3l1*	JM8.N4		24	50	Supv3l1^tm1Wcs
	JM8.N4/Supv3l1 ^{tm1a(EUCOMM)Wtsi/+}	B08	24	70.8
*Kmt5b*	JM8 parental/Kmt5b ^{tm1a(KOMP)Wtsi/+}	C01	24	16.7	Kmt5b^tm1Wcs

*Only a low number of colonies were obtained; all were screened.

Table 1. Targeting frequencies following electroporation of insertion vectors pI_hygGFP into WT JM8 (parental or subcloned N4 or F6 lines as indicated) and heterozygous mutant tm1a/+ (knockout first) ES cells, selection in hygromycin and screening/genotyping by long range-PCR on genomic DNA.