Table 2.
Primer Sequences
| Gene symbol | 5’-Primer | 3’-Primer |
|---|---|---|
| mRNA Expression | ||
| MAT2A | 5’-GATGCCAAAGTAGCTTGTGAAACT | 5’-CGTTACAAGTCTTGTAGTCAAAACCT |
| MAT2B | 5’-GAGAACAATCTAGGAGCTGCTGT | 5’-CCTTAATTGATGGATCCAGCATTCTC |
| MTHFR | 5’-CTTGAAGGAGAAGGTGTCTGCG | 5’-ATCTCCTGTGGCACCTCCA |
| AHCY | 5’-GACCGGTATCGGTTGAAGAATGG | 5’-GGTACTTGTCTGGATGGGTCCA |
| MTR | 5’-CGCAACCCGAAGGTCTGAA | 5’-TGGTAAATGACATCAGGCTGAGTTATA |
| DNMT1 | 5’-AGAAGCTGTCCATCTTTGAT | 5’-CATAGATTGGTTTTGCTGAA |
| DNTM3A | 5’-TCTTTGATGGAATCGCTAC | 5’-GCGGTAGAACTCAAAGAAGA |
| DNTM3B | 5’- GCTCTTACCTTACCATCGAC | 5’-GAGACGAGCTTATTGAAGGT |
| TET1 | 5’-CCCGGGCTCCAAAGTTGTG | 5’-GCAGGAAACAGAGTCATT |
| TET2 | 5’-TGTGTGGCACTAGATTTCAT | 5’-AGTCTCTGAAGCCTGTTGAT |
| TET3 | 5’-CAGTGGCTTCTTGGAGTCACCTC | 5’-GGATGGCTTTCCCCTTCTCTCC |
| GADD45B | 5’-ATTGCAACATGACGCTGGAA | 5’-CTGTCTGGGTCCACATTC |
| APOBEC3C | 5’-TGTATCCAGGCACATTCTACTTC | 5’-AACTGAGCGGCGCTTTAT |
| GABRD | 5’-CGACATGGACCTGGCCAAATA | 5’-CCTTCTGCTTCTTCCTGTAGTCG |
| GABRA6 | 5’-ACCTGGACTGATGAGAGGTTGAAG | 5’-CAGAAAGACACCTGGGAAAGAATG |
| GABRA1 | 5’-ACAACACTTACGCTCCAACA | 5’-TTTCGGGCTTGACCTCTTTAG |
| GAD1 | 5’-AGGCAATCCTCCAAGAACCT | 5’-GGTGGAGCGATCAAATGTCT |
| SRD5A1 | 5’-GGCGCTTCTCTATGGACTTT | 5’-CAACCTCCATTTCAGCGTATTTAG |
| AIF1 | 5’-CTGAAACGAATGCTGGAGAAAC | 5’-GAGAAAGTCAGGGTAGCTGAAC |
| ENO2 | 5’-CTGATCCTTCCCGATACATCAC | 5’-CTGGTCAAATGGGTCCTCAA |
| B2M | 5’-CTATCCAGCGTACTCCAAA | 5’-GCTCCACTTTTTCAATTCTC |
| ACTB | 5’-CTCCCTGGAGAAGAGCTAC | 5’-GATCCACACGGAGTACTTG |
| GAPDH | 5’-CGAGATCCCTCCAAAATCAA | 5’-TTCACACCCATGACGAACAT |
| Methylated DNA Immunoprecipitation | ||
| TSH2B | Promoter region, Diagenode (Denville, NJ), Catalog-#C17011041 | |
| GAPDH | TSS region, Diagenode (Denville, NJ), Catalog-#pp-1047–050 | |
| GABRD | ||
| -633-414bp | 5’-AAGGCAGGAGAGTGTGAGGCC | 5’-TGCTTCCAAAACCCTCCTGCCTT |
| -346-153b | 5’-CCAGAAGCAGCCACGCAGA | 5’-GGCAGCTCTCCCTGGCC |
| GABRA6 | ||
| -215-111bp | 5’-GGAATCCAAGAGGGTCACAATAG | 5’-TTCCAATAGGCTCAGCGTTTAT |
| -37 + 63bp | 5’-GGCAGTGGATTTCTTCCTTCTA | 5’-GTTGGGAAAGGAGAGTCTGAAG |
| +215 + 325bp | 5’-AGGCAAACAAGGAACAGAGATA | 5’-AAATGCAGAATTCACCCTCCTA |