Fig. 1.

Intratumoral molecular subtype heterogeneity in GBM is directly influenced by the tumor microenvironment. Transcriptional expression profiles were obtained from the Ivy GAP database,¹¹ which used laser capture microdissection to enrich geographically distinct regions within individual patient derived tumors. This RNA-seq dataset, which included 90 structural regions obtained from 37 individual tumors, was molecularly subtyped using gene expression values of 840 transcripts as described by Verhaak et al. (A) Heat map and (B) graphical depiction of molecular subtypes identified in the individual structural regions. Subtypes: proneural (PN), neural (N), classical (CL), mesenchymal (M). Structural regions: leading edge (LE), infiltrating tumor (IT), cellular tumor (CT), perinecrotic zone (PNZ), pseudopalisading cells around necrosis (PAN). Numbers of samples comprising each structural region are included in parentheses.