Fig. 2.

PCA of RNA-seq profiles in the context of their structural regions demonstrates limited intertumoral heterogeneity in GBM. (A) PCA was performed using the entire RNA-seq profile of individual tumor regions, including the infiltrating tumor (LE + IT), perinecrotic region (PNZ, PAN), and the central tumor core. Symbols identify tumors with all 3 regions represented. (B, C) VIP analysis was performed to identify the most important genes differentiating individual regions (red: upregulation, green: downregulation). IT: infiltrative tumor; CT: cellular tumor; PN: perinecrotic region.