Table 9.
Genes encoding predicted polysaccharide degrading enzymes in the MB2003 genome
| Locus tag | Name | Annotation | Size (aa) | CAZya | Binding domains |
|---|---|---|---|---|---|
| bhn_I2518 | bga2A | β-galactosidaseb | 1034 | GH2 | |
| bhn_I0827 | bga2C | β-galactosidaseb | 714 | GH2 | |
| bhn_I1587 | bga2B | β-galactosidaseb | 825 | GH2 | |
| bhn_I0200 | gh2B | glycoside hydrolase family 2b | 641 | GH2 | |
| bhn_I1127 | gh2A | glycoside hydrolase family 2b | 912 | GH2 | |
| bhn_I1849 | gh2C | glycoside hydrolase family 2b | 776 | GH2 | |
| bhn_III062 | bgl3A | β-glucosidaseb | 803 | GH3 | |
| bhn_I0707 | bgl3B | β-glucosidaseb | 808 | GH3 | |
| bhn_I0180 | bgl3C | β-glucosidaseb | 671 | GH3 | |
| bhn_I0706 | bgl3D | β-glucosidase b | 982 | GH3 | C-terminal TMH |
| bhn_I0189 | xyl3A | β-xylosidaseb | 707 | GH3 | |
| bhn_I1640 | bhx3A | β-N-acetylhexosaminidase b | 427 | GH3 | |
| bhn_I1693 | cel5C | endo-1,4-β-glucanase b | 543 | GH5 | CBM2a |
| bhn_I0165 | cel5A | endo-1,4-β-glucanase/xylanase b | 417 | GH5 | |
| bhn_I1756 | xyn8A | reducing end xylose-releasing exo-oligoxylanaseb | 383 | GH8 | |
| bhn_I0834 | cel9B | cellodextrinaseb | 552 | GH9 | CelD |
| bhn_I0568 | xyn10B | endo-1,4-β-xylanase b | 425 | GH10 | |
| bhn_I0169 | xyn10A | endo-1,4-β-xylanase b | 451 | GH10 | |
| bhn_I1458 | glgB2 | 1,4-α-glucan branching enzymeb | 824 | GH13 | CBM48 |
| bhn_I0053 | glgB1 | 1,4-α-glucan branching enzymeb | 663 | GH13 | CBM48 |
| bhn_I2702 | amy13A | α-amylaseb | 697 | GH13 | CBM34 |
| bhn_I0634 | amy13B | α-amylase b | 536 | GH13 | |
| bhn_I1680 | amy13C | α-amylaseb | 434 | GH13 | |
| bhn_I0669 | amy13D | α-amylaseb | 511 | GH13 | |
| bhn_I1153 | glgX1 | glycogen debranching enzymeb | 726 | GH13 | CBM48 |
| bhn_I1315 | glgX2 | glycogen debranching enzymeb | 648 | GH13 | |
| bhn_I0652 | suc13P | sucrose phosphorylaseb | 553 | GH13 | |
| bhn_I2526 | chi18A | chitinase b | 567 | GH18 | |
| bhn_I1254 | lyc25A | lysozyme b | 362 | GH25 | |
| bhn_III074 | lyc25B | lysozyme b | 515 | GH25 | |
| bhn_I0191 | lyc25C | lysozyme b | 561 | GH25 | |
| bhn_I1763 | lyc25D | lysozyme b | 242 | GH25 | |
| bhn_I0527 | lyc25E | lysozymeb | 1213 | GH25 | Big2 (×2) |
| bhn_I1287 | aga27A | α-galactosidaseb | 577 | GH27 | |
| bhn_I0082 | gh27A | glycoside hydrolase family 27b | 442 | GH27 | |
| bhn_I1952 | pg128A | polygalacturonaseb | 531 | GH28 | |
| bhn_I2679 | pgl28B | polygalacturonaseb | 519 | GH28 | |
| bhn_I1087 | fuc29A | α-L-fucosidaseb | 475 | GH29 | |
| bhn_I2734 | gh30A | glycoside hydrolase family 30 b | 575 | GH30 | |
| bhn_I1581 | gh31A | glycoside hydrolase family 31b | 756 | GH31 | |
| bhn_I2191 | gh31C | glycoside hydrolase family 31b | 674 | GH31 | |
| bhn_I0283 | gh31B | glycoside hydrolase family 31b | 635 | GH31 | |
| bhn_I0582 | scr32A | sucrose-6-phosphate hydrolaseb | 493 | GH32 | |
| bhn_I0826 | bga35A | β-galactosidaseb | 622 | GH35 | |
| bhn_I1817 | bga35B | β-galactosidaseb | 735 | GH35 | |
| bhn_I0644 | aga36A | α-galactosidaseb | 782 | GH36 | |
| bhn_I1583 | aga36B | α-galactosidaseb | 620 | GH36 | |
| bhn_I1945 | aga36C | α-galactosidaseb | 730 | GH36 | |
| bhn_I0086 | man38A | α-mannosidaseb | 1053 | GH38 | |
| bhn_III010 | bga42A | β-galactosidaseb | 673 | GH42 | |
| bhn_I0167 | xsa43A | xylosidase/arabinofuranosidase b | 543 | GH43 | CBM6 |
| bhn_I0981 | xsa43B | xylosidase/arabinofuranosidaseb | 301 | GH43 | |
| bhn_I2037 | xsa43C | xylosidase/arabinofuranosidaseb | 302 | GH43 | |
| bhn_I2111 | xsa43D | xylosidase/arabinofuranosidaseb | 517 | GH43 | |
| bhn_I2735 | xsa43E | xylosidase/arabinofuranosidaseb | 352 | GH43 | |
| bhn_I0032 | xsa43G | xylosidase/arabinofuranosidaseb | 312 | GH43 | |
| bhn_I0164 | xsa43F | xylosidase/arabinofuranosidase and esteraseb | 925 | GH43 | |
| bhn_I1509 | arf51C | α-L-arabinofuranosidaseb | 630 | GH51 | |
| bhn_I2228 | arf51A | α-L-arabinofuranosidaseb | 502 | GH51 | |
| bhn_I0010 | arf51B | α-L-arabinofuranosidaseb | 504 | GH51 | |
| bhn_I0670 | agn53A | arabinogalactan endo-1,4-β-galactosidase b | 439 | GH53 | |
| bhn_I0183 | agu67A | α-D-glucuronidaseb | 662 | GH67 | |
| bhn_I2177 | mal77A | 4-α-glucanotransferase b | 506 | GH77 | |
| bhn_I0697 | ugl88A | unsaturated glucuronyl hydrolaseb | 385 | GH88 | |
| bhn_I2381 | ugl88B | unsaturated glucuronyl hydrolaseb | 383 | GH88 | |
| bhn_I2196 | cbp94A | cellobiose phosphorylaseb | 814 | GH94 | |
| bhn_I1582 | gh95A | glycoside hydrolase family 95b | 734 | GH95 | |
| bhn_I2548 | gh105A | unsaturated rhamnogalacturonyl hydrolaseb | 349 | GH105 | |
| bhn_I0090 | gh105B | unsaturated rhamnogalacturonyl hydrolaseb | 363 | GH105 | |
| bhn_I2549 | gnpA | D-galactosyl-β-1-4-L-rhamnose phosphorylaseb | 722 | GH112 | |
| bhn_I0185 | gh115A | α-glucuronidaseb | 947 | GH115 | |
| bhn_I1083 | xyl120A | xylosidaseb | 861 | GH120 | |
| bhn_I1738 | xyl120B | xylosidaseb | 664 | GH120 | |
| bhn_III076 | est1A | feruloyl esterase b | 351 | CE1 | |
| bhn_I1244 | est2A | acetyl-xylan esteraseb | 372 | CE2 | |
| bhn_III070 | est4A | polysaccharide deacetylaseb | 207 | CE4 | |
| bhn_I0843 | est4C | polysaccharide deacetylase b | 280 | CE4 | |
| bhn_I0666 | nagA | N-acetylglucosamine-6-phosphate deacetylaseb | 371 | CE9 | |
| bhn_I1609 | est12A | carbohydrate esterase family 12b | 584 | CE12 | |
| bhn_I1927 | est12B | carbohydrate esterase family 12b | 244 | CE12 | |
| bhn_I1926 | pl11A | polysaccharide lyaseb | 746 | PL11 | |
| bhn_I0657 | glgP1 | glycogen phosphorylaseb | 769 | GT35 | |
| bhn_I2673 | glgP2 | glycogen phosphorylaseb | 824 | GT35 | |
| bhn_I1848 | – | carbohydrate binding protein b | 983 | CBM2a (×1), CBM6 (×6) |
aCAZy descriptions and classifications compiled from the CAZy database [68]. bIndicates homologues in the B. hungatei JK615T draft genome. Genes encoding predicted secreted polysaccharide degrading enzymes are in bold