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. 2017 Dec 4;17:87. doi: 10.1186/s12896-017-0405-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Purification of RANK-N and glycosylation analysis. a The growth curve of Pichia pastoris in a shake flask at 30 °C. b SDS-PAGE analysis of purified RANK-N protein. M: molecular weight marker; Lane 1: Induction by methanol at 96 h; Lane 2: Flow-through of ultrafiltration; Lane 3: Sample after ultrafiltration; Lane 4: Fraction 1 of Sephadex G-50 gel chromatography; Lane 5: Flow-through from Q-Sepharose-FF chromatography; lanes 6–9: Fraction 1 of Q-Sepharose Fast Flow chromatography. c Treatment of the protein with PNGase F. Lane 1: molecular weight marker; Lane 2: RANK-N; Lane 3: RANK-N+ PNGase F, arrow indicates PNGase F