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. 2017 Dec 4;17:87. doi: 10.1186/s12896-017-0405-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Biacore T100 analysis of RANKL/RANK-N binding. Concentrations of RANK-N (from 32.5 to 1000 nM) were passed over a CM5 chip that was coated with RANKL. The data are plotted as the steady-state response (in RU) vs RANK-N concentration and fit to a rearranged Hill equation, resulting in a K_D of 2.62×10⁻⁶ M