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. 2017 Dec 4;216(12):4235–4254. doi: 10.1083/jcb.201701108

Figure 2.

Figure 2.

Morphology of intra-PV host vesicles. (A and B) HeLa cells transiently transfected with GFP-Rab1A (green) were infected with RFP-RH for 24 h, stained with DAPI (blue), and processed for CLEM. An overlay of a fluorescent image with its corresponding EM image is shown for two different PVs. Magnified EM serial sections of the boxed regions are shown (A1 and A1′, B1 and B1′, and B2 and B2′; bars, 100 nm). Black arrow in B1 shows attachment point of the vesicles to the PVM. Structures corresponding to the GFP-Rab1A foci are indicated with green arrowheads. Bars, 500 nm. (C) Infected VERO cells were incubated with LDL adsorbed to gold particles for 20 h and processed for TEM. Intra-PV structures containing gold particles are indicated with black arrowheads. Bar, 100 nm. (D) Infected (24 h), GFP-Rab11A–expressing VERO cells were immunogold labeled with antibodies to GFP. Arrowheads indicate GFP-Rab11A vesicles. The diagram corresponds to the immuno-EM image with host GFP-Rab11A vesicles (blue), the PVM (brown), and the parasite’s plasma membrane (PPM; black). Bar, 100 nm. (E) Distribution of intra-PV structures containing vesicles unlabeled or single or double labeled with gold particles. Bars, 50 nm. hc, host cell; P, parasite.