Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 4;216(12):4235–4254. doi: 10.1083/jcb.201701108

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Romano et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 9. — Distribution of host derived ceramides in the PV and parasites. (A and B) HFF cells infected with RH or Δgra2Δgra6 parasites (20 h) were incubated with BODIPY TR C5-ceramide for 5 min (A; bars, 3 µm) or 40 min (B; bars, 7 µm) and fixed and viewed by fluorescence microscopy. Individual z-slices are shown. For the 5-min treatment, PVs containing eight parasites were placed into categories based on their staining pattern as described in Materials and methods. Arrowheads in A show filamentous structures connecting the PVM with parasites. *, P < 0.005. (C) HeLa cells were infected with RH or Δgra2Δgra6 parasites (20 h) and immunostained for TgGRA7 to detect PVM projections (PVMPs). Total intensity projections of z-stacks and percentage of the PV population with PVMPs (mean ± SD) are shown. *, P < 0.0001. Bars, 7 µm. (D) Hypothetical model of host vesicles internalized into the PV in WT (left cartoons) and Δgra2Δgra6 parasites. H.O.S.T., host organelle–sequestering tubulostructures.