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. 2017 Dec 4;216(12):4041–4052. doi: 10.1083/jcb.201703096

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© 2017 Ribet et al.

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Figure 3. — Mapping of human septin SUMOylation sites. (a) Schematic representation of HA-tagged WT and mutant SEPT2, SEPT6, SEPT7, SEPT9, and SEPT11. Black arrowheads indicate K to R mutations (KRn, K to R mutant in all N-terminal lysines; KRn+m, K to R mutant in all N-terminal and central domain lysines; KRc, K to R mutant in all C-terminal lysines). (b) HeLa cells were cotransfected with WT His₆-SUMO1 and WT or mutant HA-tagged septins. Cell lysates were then subjected to denaturing His pull-down, and the presence of SUMOylated septins was assayed using anti-HA antibodies. Input fractions are shown as controls.