Figure 7.

The interaction between cTAGE5 and Sec22b is important for proinsulin processing. (A) MIN6 cells were infected with lentivirus expressing scramble (shCtrl) or shcTAGE5, together with lentivirus expressing vector, human cTAGE5 full-length, or mutants (cTAGE5 △CC1 and cTAGE5 △CC2). Proinsulin processing was analyzed as in Fig. 5 A. (B) Pancreas sections stained with insulin (red) and Flag-cTAGE5 (green). The arrow shows β cell in islets; the arrowhead shows non–β cells in islets. TG, hcTAGE5 transgenic mice. Bar, 50 µm. (C) Pancreas sections stained with insulin (red) and DAPI (blue). Bar, 50 µm. (D) IPGTT was performed. Area under the curve (AUC) shown at right (n = 3 for all mouse lines). All data are means ± SEM (Student’s t test) from more than two independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.