Figure 2. Treg cell-specific cell surface marker expression in RORγt+CCR6+ Treg cells.
(A) Seven days after MOG/CFA s.c. immunization, dLNs were isolated from C57BL/6 mice. Cell surface marker expression in RORγt+CCR6+CD4+Foxp3+ cells (Solid) was compared with that in RORγt−CCR6−CD4+Foxp3+ cells (Dashed). (B) Seven days after MOG/CFA immunization, RORγt expression in each CD4+Foxp3+ subsets in dLNs was analyzed after gating based on ICOS and CCR6 expressions. (C) Ki-67 expression in RORγt+CCR6+ (Solid) or RORγt−CCR6− (Dashed) cells was analyzed. Data are representatives of at least two independent experiments. See also Figure S1.