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. 2017 Dec 4;10:597. doi: 10.1186/s13071-017-2545-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Immunohistochemical localization of E. granulosus calmodulin (EgCaM) in sections of E. granulosus. Anti-recombinant EgCaM (rEgCaM) rabbit IgG was used as the primary antibody and fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG was used as the secondary antibody to detect the native protein in protoscoleces, the germinal layer, and adult worms. The nuclear DNA was stained with 2-(4-amidinophenyl)-1H–indole-6-carboxamidine (DAPI). Pre-immune rabbit sera was used as negative control. Images of protoscoleces are magnified at ×400; germinal layer and adult worm are magnified at ×200. Abbreviations: LL, laminated layer; GL, germinal layer; Teg, tegument; PR, parenchymal region