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. 2017 Dec 4;10:597. doi: 10.1186/s13071-017-2545-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Relative expression levels of recombinant E. granulosus calmodulin mRNA (rEgCaM) in PSCs treated with H₂O₂ for different times. The mRNA expression of rEgCaM in PSCs treated with 5 mM H₂O₂ for 0, 1, 2, 3, 4, 5 and 6 h were assessed by qPCR analysis and normalized to the levels of the β-actin gene. Data are presented as the mean ± SD of triplicate experiments. Statistically significant differences between the 0 h group (as the control) and the other groups were determined using Student’s t-test (2 h: t ₍₇₎ = 3.832, P = 0.005; 6 h: t ₍₇₎ = -2.792, P = 0.000) (*P < 0.05*)